| 质粒名称: | pLenti 4.1 Ex miR200c-141 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 35534 |
| 存储实验室: | Greg Goodall |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti 4.1Ex |
| 载体出品公司: | Scott Hammond |
| 空载体大小: | 8749 bp |
| 完整质粒大小: | 9439 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | miR200c-141 genomic cluster、MIRN200C、MIRN141 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 690 bp |
| 基因突变: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
| GenBank编号 | 406985 406933 |
| 启动子: | CMV |
| 标签/融合蛋白: | GFP (C terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI (not destroyed) |
| 3’克隆位点: | AvrII (destroyed during cloning) |
| 5’测序引物 | 未知 |
| 3’测序引物 | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | |
| 参考文献部分: | |
买家导航
pLenti 4.1 Ex miR200c-141载体质粒基本信息
pLenti 4.1 Ex miR200c-141质粒图谱载体图谱和pLenti 4.1 Ex miR200c-141载体序列质粒序列多克隆位点信息
pLenti 4.1 Ex miR200c-141质粒载体简介
pLenti 4.1 Ex miR200c-141质粒序列载体序列
LOCUS pLenti 4.1 Ex miR200c-141 9439 bp DNA circular SYN 14-APR-2025
DEFINITION 3rd generation lentiviral vector.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9439)
AUTHORS Gregory PA, Bert AG, Paterson EL, Barry SC, Tsykin A, Farshid G,
Vadas MA, Khew-Goodall Y, Goodall GJ
TITLE The miR-200 family and miR-205 regulate epithelial to mesenchymal
transition by targeting ZEB1 and SIP1.
JOURNAL Nat Cell Biol. 2008 May;10(5):593-601. Epub 2008 Mar 30.
PUBMED 18376396
REFERENCE 2 (bases 1 to 9439)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell
Biol."; date: "2008-05"; volume: "10(5)"; pages: "593-601. Epub 2008
Mar 30"
COMMENT Sequence Label: pLenti 4.1 Ex miR200c-141
FEATURES Location/Qualifiers
source 1..9439
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1686..1919
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2104..2148
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2446..2563
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
polyA_signal 2637..2844
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind 2833..2850
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
CDS complement(join(2863..3576,3577..3579,3580..3582))
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS complement(join(2866..3576,3577..3579,3580..3582))
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2908..2929)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 3257..3276
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3516..3537
/label=EGFP-N
/note="EGFP, reverse primer"
regulatory 3579..3588
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
promoter complement(4526..4729)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer 4730..5033
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
protein_bind complement(5083..5116)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
promoter 5139..5638
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(5174..5197)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 5571..5590
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 5659..6258
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5659..5678)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6155..6175
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 6328..6916
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6381..6401)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6799..6810)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6919..6935)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6920..6936)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 7445..7625
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(7687..8275)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7767..7786)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(8446..9237,9238..9306))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9069..9088
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9307..9411)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
2581 cagcagagat ccagtttggt tagtaccggg cccgctctag aatagagccc accgcatccc
2641 cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc
2701 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
2761 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
2821 atggctggca actagaaggc acagtcgagg ctggcggccg ctttacttgt acagctcgtc
2881 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
2941 ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag
3001 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
3061 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
3121 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
3181 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
3241 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
3301 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
3361 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
3421 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
3481 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
3541 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcgc ccgggggatc
3601 tagctgtgga aaaaaaaggg acaggataag tatgatattg tcaaggaaac cctgaattgc
3661 tcgagttaat taaggccacc cgggcctgca ggattcgaat cggcgcgcct aggaggggtg
3721 aaggtcagag gttgttggtc agtagtcctc catggtcttc agggctccct gaaggttact
3781 gccgagagaa cccacccggg agccatcttt accagacagt gttaggagct tcacaattag
3841 accatccaac actgtactgg aagatggacc cagggccggc cgacagagaa ctacggtgcg
3901 cgctcaccag ttgctacagg ggaccctggc cacaggccag gtgaggtctc ttgagagggg
3961 gcgagccaac cccaagctca aggtcgactg tgggttctgg atccaccagg tgacaaatcg
4021 cactgggtgg gaggaggggc tggggcccat tgtgtccctt agtggcagcc agccaacaag
4081 aaccacccct tgtgcaacgc tctcagctca agacgagggg cttccggggt gcgtgggaag
4141 tggggagggg gctgcagcca gctaagggct ggggacctga ggcgatggat gttgctgaca
4201 cagggacagg ggcctccatc attacccggc agtattagag actcccaacc gcacccaaac
4261 actgctgggt aagacgaggg cccccgccca gcccgcccac cttgggtcag gcagcttcag
4321 gcccaggatc cctgcggaaa agctgcagat ccctggctcc catcccccca ccacccgagt
4381 ccctggggac acttcctggt gagccctgaa ttcgtcgaga tttaaataga tctccactta
4441 cctgggatct gagtccggta gcgctagcaa taacttcgta taatgtatgc ttatcgaagt
4501 tatggatctg acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc
4561 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
4621 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
4681 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
4741 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
4801 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
4861 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
4921 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
4981 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
5041 ccatatatgg gctatgaact aatgaccccg taattgatta ctataacttc gtatagcata
5101 cattatacga agttatctta attaactaca attctaccgg gtaggggagg cgcttttccc
5161 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
5221 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
5281 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
5341 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
5401 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
5461 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
5521 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
5581 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
5641 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
5701 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
5761 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
5821 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
5881 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
5941 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
6001 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
6061 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
6121 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
6181 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
6241 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
6301 cgacaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
6361 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
6421 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
6481 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
6541 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
6601 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
6661 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
6721 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
6781 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
6841 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
6901 tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc
6961 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
7021 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
7081 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
7141 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
7201 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
7261 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
7321 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
7381 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
7441 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
7501 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
7561 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
7621 tagcagcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
7681 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
7741 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
7801 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
7861 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
7921 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
7981 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
8041 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
8101 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
8161 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
8221 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
8281 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
8341 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
8401 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
8461 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
8521 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
8581 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
8641 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
8701 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
8761 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
8821 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
8881 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
8941 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
9001 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
9061 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
9121 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
9181 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
9241 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
9301 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
9361 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
9421 ccgaaaagtg ccacctgac
//
pLenti 4.1 Ex miR200c-141载体图谱质粒图谱pdf版和pLenti 4.1 Ex miR200c-141载体序列质粒序列等相关资料下载
pLenti 4.1 Ex miR200c-141质粒载体应用举例
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